Systemic lipid peroxidation profile from patients with breast cancer changes according to the lymph nodal metastasis status

Stefania Tagliari de Oliveira1, Monica Pavaneli Bessani1, Thalita Basso Scandolara1,2, Janaína Carla Silva1, Aedra Carla Bufalo Kawassaki1, Pâmella Aparecida Ferreira Fagotti1,4, Vitor Teixeira Maito1, Janoário Athanazio de Souza1,3, Daniel Rech1,3, and Carolina Panis1,4

1 Laboratory of Tumor Biology, State University of West Paraná, Unioeste, Francisco Beltrão, Paraná, Brazil

2 Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

3 Francisco Beltrão Cancer Hospital, Ceonc, Francisco Beltrão, Paraná, Brazil

4 State University of Londrina, Londrina, Paraná, Brazil

Correspondence to:

Carolina Panis, email: [email protected]

Keywords: breast cancer; lipid peroxidation; prognosis; lymph nodal metastasis; tumor subtypes

Received: December 01, 2021     Accepted: February 21, 2022     Published: February 24, 2022


Metastasis is the leading cause of cancer death. Considering that lymph nodes are the major pathway for cancer spreading and that the metastatic process is under oxidative stress effects, this study aims to evaluate the differential lipid peroxidation profile in the blood of breast cancer patients regarding their lymph nodal status (LN). A total of 105 women diagnosed with breast cancer were included before chemotherapy started. LN was determined by assessing the histopathological analysis of patients’ biopsies, and groups were categorized according to the presence (LN+, n = 48) or absence (LN−, n = 57) of metastases. Lipid peroxidation profiles (LPO) were determined in blood by high-sensitivity chemiluminescence. After patients’ categorization in groups according to their clinicopathological features, LN− patients aged over 50 years presented significantly lower LPO when compared to those under 50 years. Further, LN− patients carrying HER2 positive tumors presented augmented LPO when compared to patients bearing luminal B or triple-negative tumors. LN+ group also had reduced LPO when presented intratumoral clots. The significant contribution of this study was to show that LPO correlates with specific clinical features of patients with breast cancer according to their LN status and that such profile is significantly affected by the presence of metastases.

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